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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 <t>VA4</t> cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
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Image Search Results


TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 VA4 cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.

Journal: Experimental & Molecular Medicine

Article Title: TFAP2C-mediated upregulation of TGFBR1 promotes lung tumorigenesis and epithelial–mesenchymal transition

doi: 10.1038/emm.2016.125

Figure Lengend Snippet: TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 VA4 cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.

Article Snippet: The human NSCLC cell lines, NCI-H292 and NCI-H838, and the human normal lung cell lines, MRC5 and WI-26 VA4, were acquired from the American Type Culture Collection (ATCC, Manassas, VA, USA), authenticated and maintained in early passages, no more than 6 months after receipt from ATCC.

Techniques: Binding Assay, Knockdown, Small Interfering RNA, Expressing, Quantitative RT-PCR, Western Blot, Control